Ex-Vivo Expansion of Fetal Cells Isolated from Maternal Circulation
نویسندگان
چکیده
منابع مشابه
Ex vivo Expansion and Differentiation of Mesenchymal Stem Cells from Goat Bone Marrow
Objective(s) Mesenchymal stem cells (MSCs) from large animals as goat which is genetically more closely related to human have rarely been gained attentions. The present study tried to isolate and characterize MSCs from goat bone marrow. Materials and Methods Fibroblastic cells appeared in goat marrow cell culture were expanded through several subcultures. Passaged-3 cells were then different...
متن کاملex vivo expansion and differentiation of mesenchymal stem cells from goat bone marrow
objective(s) mesenchymal stem cells (mscs) from large animals as goat which is genetically more closely related to human have rarely been gained attentions. the present study tried to isolate and characterize mscs from goat bone marrow. materials and methods fibroblastic cells appeared in goat marrow cell culture were expanded through several subcultures. passaged-3 cells were then differentiat...
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Large-scale expansion of highly functional adult human mesenchymal stem cells (aMSCs) remains technologically challenging as aMSCs lose self renewal capacity and multipotency during traditional long-term culture and their quality/quantity declines with donor age and disease. Identification of culture conditions enabling prolonged expansion and rejuvenation would have dramatic impact in regenera...
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Cholesterol is required for fetal development. Data obtained from recent studies in humans, rodents, and cell cultures showed that circulating maternal cholesterol can affect fetal metabolism and sterol accretion. Recent studies in our laboratory showed that the efflux of cholesterol from the basolateral side of the placental cells and the secretion of cholesterol from endodermal yolk sac cells...
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In this study, we described the modelling of the expansion/maintenance of human hematopoietic stem/progenitor cells from adult human bone marrow. CD 34(+)-enriched cell populations from bone marrow were cultured in the presence and absence of human stroma in serum-free media containing bFGF, SCF, LIF and Flt-3 ligand for several days. The cells in the culture were analysed for expansion and phe...
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ژورنال
عنوان ژورنال: Biomedical and Pharmacology Journal
سال: 2017
ISSN: 0974-6242,2456-2610
DOI: 10.13005/bpj/1268